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These protein nanodisks are similar to the minivector DNAs developed by Baylor College of Medicine in that they have improved transfection efficiency and increased serum survival. http://www.sciencedaily.com/releases/2011/01/110111132529.htm

This study evaluated prophylactic and therapeutic effects of the IL-13Rα2 cDNA vaccination in syngeneic animal models of melanoma, sarcoma and breast cancer cells expressing IL-13Rα2 to prime the immune system. CTL activity and IFN-γ release was measured in mice vaccinated … Continue reading →

This study confirms that enhanced cellular immune responses result in mice via chimaeric Gag VLPs incorporating GagRT and GagTN as vaccine boost candidates to a pVRCgrttnC DNA priming vaccine for HIV-1. The DNA prime/mixed VLP boost vaccination increased the cumulative … Continue reading →

A plasmid is extra chromosomal DNA that is not incorporated in the main helix of genomic DNA. Plasmids occur in nature and can be found everywhere – in plants, insects, and bacteria. Scientists have discovered various applications for plasmids including … Continue reading →

Baylor College of Medicine, in conjunction with The Methodist Hospital Research Institute, has developed a way to use minivector DNAs in gene therapy. These reduced sized DNA vectors (as small as 300bp) have high gene silencing capabilities and high serum … Continue reading →

Cell banking is essential for a biological product manufactured by the fermentation process, such as plasmid DNA. Particular emphasis is given to cGMP cell banks by following the regulatory standards (21CFR610). But how do you generate high quality cGMP cell banks, … Continue reading →

As a QA Manager for a plasmid manufacturer, I am always looking for innovative ways to maintain a focus on GMP and help employees understand its importance. GMP training accomplishes this, but it only occurs once or twice a year in … Continue reading →

Arguably, the most challenging step in plasmid manufacturing is cell lysis. Thousands of times larger than a typical small molecule pharmaceutical API, a plasmid is a large circular molecule that most of our clients prefer in the supercoiled form. Several things … Continue reading →

One of the major challenges of plasmid manufacturing is cleaning validation. To avoid excess costs and time, disposable technology can decrease validation needs for both the cleaning and sterilization processes of fixed equipment systems. Disposable materials for use in pharmaceutical … Continue reading →

Most companies struggle with how to make GMP training more fun and exciting. As the Quality Assurance director of a company that provides contract plasmid manufacturing, it seems almost impossible for me to find time to be creative. This year … Continue reading →